How do i differenciate a 64 bit slot from a 32 bit slot

The 64-bit PCI connector can be distinguished from 32-bit by being longer, and from PCI-X by having three segments, with the one in the middle much shorter than the others. PCI-X slots can be distinguished from 64-bit PCI as the small segment is first, instead of in the center.

64 bit versus 32 bit slots

3.3V 64-bit PCI slot has the small segment first as well.
There shouldn't be any significant physical differences between the slots. The newer PCI cards - 3.3V will fit (and work) in a PCI-X slot, and PCI-X cards will fit in an older PCI slot (as long as the voltage - 3.3V - matches). The standards are backwards compatible with the slowest connected card determining the bus speed.
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